Micropropagation is an artificial process of producing plants vegetatively through tissue culture or cell culture technique. Tissue culture or cell culture techniques are now being used for effective vegetative propagation of several horticulture and agricultural crops.
In recent years, the technique of tissue culture is increasingly employed to raise a large number of plantlets from a small tissue.
Method Of Micropropagation
The method of producing plants vegetatively through tissue culture involves five major stages.
|Stage 0||Selection of explant|
|Stage 1||Culturing the explant|
|Stage 2||Multiplication of explant shoot|
|Stage 3||Rooting of the explant shoot|
|Stage 4||Transfer of plantlets to the greenhouse|
This is the first stage of micropropagation, in this stage we select the stock plant and grow it under a suitable condition.
In this stage we took out the explant from the stock plant, wash it and transfer it to the culture medium for proper growth.
In this stage multiplication of shoot or somatic embryo formation is take place inside the culture medium.
In this stage, we transferred the shoot to the nutritive medium for the development of root or we can directly transfer it into the soil for root development.
This is the final stage of the propagation, in this stage plantlets are now well grown and transferred it to the greenhouse for control condition of temperature and humidity.
Examples of Micropropagation
Advantage’s of Micropropagation
- Through this method we get our desired product at very high rate.
- Large quantities of identical plant can be obtain from single plant tissue.
- The small size plantlets can be stored and transported easily.
- Through this method sterile hybrid can be grown even if vegetative propagation is not possible in crop.
- Through this method we can obtain virus free plants.