Micropropagation – Definition and It’s Technology – NeetHelp

Micropropagation is an artificial process of producing plants vegetatively through tissue culture or cell culture technique. Tissue culture or cell culture techniques are now being used for effective vegetative propagation of several horticulture and agricultural crops.

In recent years, the technique of tissue culture is increasingly employed to raise a large number of plantlets from a small tissue.

Method Of Micropropagation

The method of producing plants vegetatively through tissue culture involves five major stages.

StageMethod Involove
Stage 0Selection of explant
Stage 1Culturing the explant
Stage 2Multiplication of explant shoot
Stage 3Rooting of the explant shoot
Stage 4Transfer of plantlets to the greenhouse

Stage 0

This is the first stage of micropropagation, in this stage we select the stock plant and grow it under a suitable condition.

Stage 1

In this stage we took out the explant from the stock plant, wash it and transfer it to the culture medium for proper growth.

Stage 2

In this stage multiplication of shoot or somatic embryo formation is take place inside the culture medium.

Stage 3

In this stage, we transferred the shoot to the nutritive medium for the development of root or we can directly transfer it into the soil for root development.

Stage 4

This is the final stage of the propagation, in this stage plantlets are now well grown and transferred it to the greenhouse for control condition of temperature and humidity.

stages of micropopagation

Examples of Micropropagation

  • Orchids
  • Carnation
  • Gladiolus
  • Chrysanthemum
  • Potato
  • Tapioca
  • Sugarcane

Advantage’s of Micropropagation

  1. Through this method we get our desired product at very high rate.
  2. Large quantities of identical plant can be obtain from single plant tissue.
  3. The small size plantlets can be stored and transported easily.
  4. Through this method sterile hybrid can be grown even if vegetative propagation is not possible in crop.
  5. Through this method we can obtain virus free plants.

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